Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + EGFR (L858R) Kinase Enzyme System
Recombinant human EGFR (L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega ADP-Glo™ Kinase Assay + VRK2 Kinase Enzyme System
Recombinant human VRK2 (1-375) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. VRK2 (also known as vaccinia related kinase 2) is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases.
Promega CDK1/CyclinA2 Kinase Enzyme System, 1mg
Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK1 is essential for completion of START, controlling event in the cell cycle required to initiate mitosis.
Promega PKC gamma Kinase Enzyme System, 1mg
Recombinant full-length human PKC gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC gamma is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
Promega ADP-Glo™ Kinase Assay with PI:3PS
The ADP-Glo™ Kinase assay and lipid kinase substrates PIP2:3PS and PI:3PS are components of the PI3K-Glo™ Class I Kinase Profiling System that are available for purchase separately.
Promega CDK6/CyclinD3 Kinase Enzyme System, 1mg
Recombinant full-length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Promega ADP-Glo™ Kinase Assay + MET (M1250T) Kinase Enzyme System
Recombinant human MET (point mutant M1250T; amino acids 956-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ALK2 Kinase Enzyme System, 10microg
ALK2 is a receptor serine/threonine kinase that is member of the ALK family and is upstream of signaling pathway involving the SMAD proteins especially SMAD1/5/8.
Promega GSK3 beta Kinase Enzyme System, 1mg
Recombinant full-length human GSK3 beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3 beta is a serine-threonine protein kinase originally identified as the kinase that phosphorylates and inhibits glycogen synthase.
Promega ADP-Glo™ Kinase Assay + PAK1/CDC42 Kinase Enzyme System
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).
Promega ADP-Glo™ Kinase Assay + IKKβ Kinase Enzyme System
Full length recombinant human IKKβ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega MLK2 Kinase Enzyme System, 1mg
Recombinant human MLK2 (1-446) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK2 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8/JNK1 and MKK4/SEK1.
Promega PKC zeta Kinase Enzyme System, 1mg
Recombinant full-length human PKC zeta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC zeta is an atypical isoform of the PKC family.
Promega ADP-Glo™ Kinase Assay + SIK Kinase Enzyme System
Recombinant human SIK (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. SIK is a protein kinase that is involved in regulating AMPK-related kinases.
Promega PAK1/CDC42 Kinase Enzyme System, 10microg
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).