Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + GRK5 Kinase Enzyme System
Recombinant full-length human GRK5 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family.
Promega ADP-Glo™ Kinase Assay + ERK2 Kinase Enzyme System
Recombinant full-length human ERK2 was expressed by E. coli cells using an N-terminal GST tag and activated by MEK1 in vitro. ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega ADP-Glo™ Kinase Assay + CDK5/p35 Kinase Enzyme System
Recombinant full-length human CDK5 and human p35 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.
Promega ADP-Glo Kinase Assay + p38 alpha Kinase Enzyme System, 1 each
Recombinant full-length human p38alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38alpha (SAPK2A) is a member of the p38 MAPK family, which are activated by various environmental stresses and proinflammatory cytokines.
Promega Caspase-Glo 1 Inflammasome Assay, 10ml
The Caspase-Glo 1 Inflammasome Assay is a homogeneous, bioluminescent method to selectively measure the activity of caspase-1, a member of the cysteine aspartic acid-specific protease (caspase) family and an essential component of the inflammasome.
Promega NAD(P)H-Glo™ Detection System
The NAD(P)H-Glo™ Detection System is a bioluminescent assay that measures the concentration of the reduced forms of nicotinamide adenine dinucleotides, NADH and NADPH, and does not discriminate between them.
Promega Glutamine/Glutamate-Glo Assay, 5ml
The Glutamine/Glutamate-Glo Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples. The assay is based on the conversion of glutamine to glutamate by Glutaminase enzyme.
Promega ENLITEN™ ATP Assay System
The ENLITEN ATP Assay System is used to measure ATP levels for the indirect detection of biocontamination.
Promega NADP/NADPH-Glo™ Assay
The NAD/NADH-Glo™ Assay is a bioluminescent, homogeneous single-reagent-addition assay for detecting total oxidized and reduced nicotinamide adenine dinucleotides and determining their ratio in biological samples or in defined enzyme reactions.
Promega ADP-Glo™ Kinase Assay + CDK9/CyclinK Kinase Enzyme System
Recombinant full-length human CDK9 and CyclinK proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family.
Promega ADP-Glo™ Kinase Assay + HIPK3 Kinase Enzyme System
Recombinant human HIPK3 (163-562) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis.
Promega ADP-Glo™ Kinase Assay + PDGFRα (T674I) Kinase Enzyme System
Recombinant human PDGFRα (Y674I) (550-end) point mutant was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega BMX Kinase Enzyme System, 10microg
Recombinant full-length human BMX was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The BMX gene encodes a non-receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells.
Promega CAMK2 alpha Kinase Enzyme System, 1mg
Recombinant full-length human CAMK2 alpha was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2 alpha is a ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family.
Promega HIPK3 Kinase Enzyme System, 1mg
Recombinant human HIPK3 (163-562) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis.